Cell Culture
Induced Pluripotent Stem Cell (iPSC) Techniques
Differentiation of iPSC-derived Neurons in 2D and 3D Brain Organoids
Differentiation of iPSC-derived Oligodendrocytes & Microglia
Genome Editing via CRISPR/Cas9
Transduction with Lentiviruses and AAV
Cultivation of Human Cell Lines and Mammalian Primary Cells
Molecular and Cell Biology
DNA/RNA Isolation
Preparation of cDNA-libraries for RNA Sequencing
PCRs (genotyping, RT-PCR, qPCR)
Molecular Cloning (incl. Gibson Assembly, Site-directed Mutagenesis)
Immunofluorescence Staining
SDS-PAGE, Western Blot
Co-Immunoprecipitation
Vesicle Isolation
Microscopy
Widefield Fluorescence Microscopy
Confocal Microscopy
Live-Cell Imaging (Calcium Imaging, Cell Tracking)
Image Processing (ImageJ, CellProfiler, Imaris)
Bioengineering
Electrochemical Sensors
CAD design & 3D Printing
Photolithography
Chip/Microneedle Fabrication
